Full list of starts for the 2013 elite men's time trial world championship in Tuscany, Italy, on Wednesday September 25.
Note that the start times are British Summer Time. Add one hour for local time.
| Start time | Number | Name | Nation |
| 12:15:00 | 79 | MUGWANYA Herbert | UGA |
| 12:16:30 | 78 | MINO Gustavo | PAR |
| 12:18:00 | 77 | JASER Nazir | SYR |
| 12:19:30 | 76 | MACHADO Tiago | POR |
| 12:21:00 | 75 | RAGONESSI Jose | ECU |
| 12:22:30 | 74 | CHOE Hyeongmin | KOR |
| 12:24:00 | 73 | MIHAYLOV Nikolay | BUL |
| 12:25:30 | 72 | LUTSENKO Alexey | KAZ |
| 12:27:00 | 71 | KONOVALOVAS Ignatas | LTU |
| 12:28:30 | 70 | ELBOURDAINY Ahmed | QAT |
| 12:30:00 | 69 | GRETSCH Patrick | GER |
| 12:31:30 | 68 | TVETCOV Serghei | MDA |
| 12:33:00 | 67 | TAMOURIDIS Ioannis | GRE |
| 12:34:30 | 66 | SARAMOTINS Aleksejs | LAT |
| 12:36:00 | 65 | RUSSOM Meron | ERI |
| 12:37:30 | 64 | POKALA Samuel | FIN |
| 12:39:00 | 63 | NECHITA Andrei | ROU |
| 12:40:30 | 62 | VASYLYUK Andriy | UKR |
| 12:42:00 | 61 | MARTINS Uri | MEX |
| 12:43:30 | 60 | SANCHEZ GIL Luis Leon | ESP |
| 12:45:00 | 59 | KOREN Kristijan | SLO |
| 12:46:30 | 58 | KONONENKO Mykhaylo | UKR |
| 12:48:00 | 57 | JURCO Matej | SVK |
| 12:49:30 | 56 | JOEAAR Gert | EST |
| 12:51:00 | 55 | SANTOS SIMOES OLIVEIRA Nelson Filipe | POR |
| 12:52:30 | 54 | JANSE VAN RENSBURG Reinardt | RSA |
| 12:54:00 | 53 | GYUROV Spas | BUL |
| 12:55:30 | 52 | EUGERT Zhupa | ALB |
| 12:57:00 | 51 | KANG Jiyong | KOR |
| 12:58:30 | 50 | ZAKARIN Ilnur | RUS |
| 13:00:00 | 49 | BODNAR Maciej | POL |
| 13:01:30 | 48 | BEWLEY Sam | NZL |
| 13:03:00 | 47 | BALIKUDEMBE Rogers | UGA |
| 13:04:30 | 46 | BAGDONAS Gediminas | LTU |
| 13:06:00 | 45 | ASADOV Elchin | AZE |
| 13:07:30 | 44 | ALBOS CAVALIERE David | AND |
| 13:09:00 | 43 | ZOIDL Riccardo | AUT |
| 13:10:30 | 42 | CHTIOUI Rafaa | TUN |
| 13:12:00 | 41 | SIUTSOU Kanstantsin | BLR |
| 13:13:30 | 40 | THOMSON Jay Robert | RSA |
| 13:15:00 | 39 | LUDVIGSSON Tobias | SWE |
| 13:16:30 | 38 | GUSEV Vladimir | RUS |
| 13:18:00 | 37 | NAVARRETE Segundo | ECU |
| 13:19:30 | 36 | DENNIS Rohan | AUS |
| 13:21:00 | 35 | ROCHE Nicolas | IRL |
| 13:22:30 | 34 | BRANDLE Matthias | AUT |
| 13:24:00 | 33 | GRABSCH Bert | GER |
| 13:25:30 | 32 | MESSINEO Leandro | ARG |
| 13:27:00 | 31 | CHEUNG King Lok | HKG |
| 13:28:30 | 30 | OYARZUN GUINEZ Carlos Ivan | CHI |
| 13:30:00 | 29 | TEKLEHAYMANOT Daniel | ERI |
| 13:31:30 | 28 | HALMURATOV Muradjan | UZB |
| 13:33:00 | 27 | GRUZDEV Dmitriy | KAZ |
| 13:34:30 | 26 | RASMUSSEN Alex | DEN |
| 13:36:00 | 25 | VANDEWALLE Kristof | BEL |
| 13:37:30 | 24 | HOLLENSTEIN Reto | SUI |
| 13:39:00 | 23 | INFANTINO ABREU Rafael | COL |
| 13:40:30 | 22 | ROY Jeremy | FRA |
| 13:42:00 | 21 | LARSSON Gustav | SWE |
| 13:43:30 | 20 | BARTA Jan | CZE |
| 13:45:00 | 19 | TERPSTRA Niki | NED |
| 13:46:30 | 18 | JUNGELS Bob | LUX |
| 13:48:00 | 17 | SERGENT Jesse | NZL |
| 13:49:30 | 16 | DE GENDT Thomas | BEL |
| 13:51:00 | 15 | WESTRA Lieuwe | NED |
| 13:52:30 | 14 | DOWSETT Alex | GBR |
| 13:54:00 | 13 | QUAADE Rasmus Christian | DEN |
| 13:55:30 | 12 | KWIATKOWSKI Michal | POL |
| 13:57:00 | 11 | TALANSKY Andrew | USA |
| 13:58:30 | 10 | KIRYIENKA Vasil | BLR |
| 14:00:00 | 9 | PINOTTI Marco | ITA |
| 14:01:30 | 8 | CASTROVIEJO NICOLAS Jonathan | ESP |
| 14:03:00 | 7 | CHAVANEL Sylvain | FRA |
| 14:04:30 | 6 | PORTE Richie | AUS |
| 14:06:00 | 5 | MALORI Adriano | ITA |
| 14:07:30 | 4 | PHINNEY Taylor | USA |
| 14:09:00 | 3 | WIGGINS Bradley | GBR |
| 14:10:30 | 2 | CANCELLARA Fabian | SUI |
| 14:12:00 | 1 | MARTIN Tony | GER |
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